rabbit anti il 1r  (R&D Systems)

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: Sequence of specific primers.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: A shows TNF-α (30 kDa) and TNF-R 1 (55 kDa), IL-1β (17 kDa), IL-1R 1 (80 kDa) and β-actin (42 kDa) immunoreactive bands, respectively. Bar graphs in B, C show significant increase in the optical density of TNF-α and IL-1β, TNF-R 1 , IL-1R 1 following hypoxic exposure when compared with the corresponding controls (* P <0.01). Panels D and E show the graphical representation of the fold changes in TNF-α and IL-1β, TNF-R 1 , IL-1R 1 mRNA, respectively as quantified by normalization to the β-actin as an internal control. Significant increase in TNF-α and IL-1β, TNF-R 1 , IL-1R 1 mRNA levels in the PWM after the hypoxic exposure is evident when compared with controls (* P <0.01). The panels F show TNF-α (30 kDa) and IL-1β (17 kDa) immunoreactive protein bands in cultured control astrocytes and at 3 h after hypoxic exposure. G is bar graph showing changes in the optical density of TNF-α and IL-1β, respectively, following hypoxic exposure. H (TNF-α and IL-1β mRNA) show the graphical representation of the fold changes quantified by normalization to the β-actin as an internal control. Significant differences in protein and mRNA levels in astrocytes after the hypoxic exposure are evident when compared with controls (* P <0.01).

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: The co-localized expression of APC with TNF-R 1 and IL-1R 1 is depicted in C and F, I and L, respectively. Note the expression of TNF-R 1 and IL-1R 1 is upregulated after the hypoxic exposure. Scale bars: A–L, 50 µm.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Astrocyte-Derived Proinflammatory Cytokines Induce Hypomyelination in the Periventricular White Matter in the Hypoxic Neonatal Brain

doi: 10.1371/journal.pone.0087420

Figure Lengend Snippet: Confocal images showing TNF-R 1 and IL-1R 1 expression (B, E, H, K, red) in primary cultured oligodendrocytes labeled with APC (A, D, G, J, green) in both control and hypoxia for 3 h. Note TNF-R 1 and IL-1R 1 immunofluroscence intensity is markedly enhanced after hypoxic exposure (E, K) in comparison with the control (B, H). Scale bars: A–L, 50 µm.

Article Snippet: Immunofluorescence labeling was carried out using primary antibodies directed against TNF-R1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat. No.sc-7895), anti-IL-1R 1 (rabbit polyclonal IgG 1∶500) (Santa Cruz Biotechnology; Cat. No.sc-689) and APC monoclonal antibody against adenomatus polyposis coli (APC) (oncogene, mouse monoclonal IgG 1∶20; Cat.No.OP80).

Techniques: Expressing, Cell Culture, Labeling